Spinning off from the popular Professor Layton puzzle games, this movie brings Layton and his trusty assistant Luke to the big screen to allow fans to enjoy more of the same. At the beginning of the film Layton receives a letter from the famed opera singer, Jenice Kaitly, inviting them to see her newest performance. She also asks Layton, Luke, and Remi for assistance in a mystery, saying her friend Melina went missing one year ago but returned later as a seven year old girl saying she had found the secret of eternal life. Naturally, the case fascinates Layton and his apprentices, so they head to the Crown Petone opera house. When they arrive, something is very strange: the pianist, Ozro Vislar talks of a game, and the winner will win the "eternal life." It is up to Layton, Luke, and Remi to solve the mystery of the Eternal Diva before it is too late.